What is the maximum time limit for separating serum or plasma from cells according to CLSI?

The maximum time limit for separating serum or plasma from cells, as per CLSI (Clinical and Laboratory Standards Institute) guidelines, is 2 hours from the time of collection. This time frame is crucial because prolonged contact of serum or plasma with cells can lead to changes in analyte concentrations due to cellular metabolism, enzymatic reactions, or hemolysis, which can affect the accuracy and reliability of test results.

By ensuring that separation occurs within this 2-hour window, laboratories can maintain the integrity of the samples and ensure that the measurements reflect the patient's true biochemical status. This understanding helps phlebotomists and laboratory personnel adhere to best practices and ensure quality control in the testing process.